Pair Name	Epigallocatechin gallate, Irinotecan
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induction-->DNA damage
Gene Regulation	Down-regulation	Expression	CCNB1	hsa891
	Down-regulation	Expression	CCND1	hsa595
	Down-regulation	Expression	CDK4	hsa1019
	Up-regulation	Expression	MAP1LC3A	hsa84557
	Up-regulation	Phosphorylation	RB1	hsa5925
	Down-regulation	Expression	TOP1	hsa7150
In Vitro Model	RKO	Colon carcinoma	Homo sapiens (Human)	CVCL_0504
	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
Result	EGCG synergizes the therapeutic effect of irinotecan through enhanced DNA damage in human colorectal cancer cells

Pair Name	Epigallocatechin gallate, Irinotecan
Disease Info	[ICD-11: 2B91]	Colorectal cancer		Investigative
Biological Phenomena	Induced-->GRP78-mediated endoplasmic reticulum stress
Gene Regulation	Down-regulation	Expression	BCL2	hsa596
	Up-regulation	Expression	GRP78	KEGG ID N.A.
	Down-regulation	Expression	ROS1	hsa6098
In Vitro Model	HCT 116	Colon carcinoma	Homo sapiens (Human)	CVCL_0291
	RKO	Colon carcinoma	Homo sapiens (Human)	CVCL_0504
In Vivo Model	The concentration of HCT116 cells in the logarithmic phase was adjusted to 2.5×10⁷/mL, and the 200 μL cell suspension was inoculated subcutaneously on the right dorsal side of the mouse. When the average tumor volume reached 100 mm3, animals were randomized into 4 groups (5 mice for each group), control (Control, normal saline, 1 time per day, ip), irinotecan (IRI, 4 mg/kg irinotecan, 2 times per week, ip), EGCG (EGCG, 5 mg/kg, 1 time per day, ip), and irinotecan in combination with EGCG (IRI + EGCG).
Result	These reults confirmed that EGCG alone or in combination with irinotecan could up-regulate the GRP78, activate ERS of colorectal cancer cells, reduce intracellular reactive oxygen species and mitochondrial membrane potential, and induce apoptosis. The mouse xenograft experiment also confirmed the synergistic effect of EGCG and irinotecan on ERS and tumor cell.EGCG can induce GRP78-mediated endoplasmic reticulum stress and enhance the chemo-sensitivity of colorectal cancer cells when coadministered with irinotecan.

Pair Name	Epigallocatechin gallate, Irinotecan
Disease Info	[ICD-11: 2A00-2F9Z]	Solid tumour or cancer		Investigative
Biological Phenomena	Inhibition-->P-gp expression
In Vivo Model	Adult, male Sprague–Dawley rats (300 ± 20 g body weight) were provided by the Laboratory Animal Center at National Yang-Ming University, the rats were initially anaesthetized with urethane 1 g/mL and α-chloralose 0.1 g/mL (1 mL/kg, i.p.), and remained anaesthetized throughout the experimental period. The femoral vein was exposed for further drug administration.
Result	EGCG was found to inhibit the transport of CPT-11 and SN-38 into the biliary elimination and their half-lives in plasma could be substantially prolonged. Based on the food-drug interaction, persons taking daily nutritional supplements should be warned of this interaction possibility.